Abstract
In this application note, a purified polyclonal antibody (IgG) is separated using Size Exclusion Chromatography (SEC) and characterized using the Viscotek SEC-MALS 20, allowing calculation of molecular weight and radius of gyration (Rg).

Introduction
Therapeutic recombinant antibodies, primarily of the Immunoglobulin G isotype (IgG) (figure 1), represent a growing proportion of biopharmaceuticals. There are an ever increasing number of approved antibody therapies available to treat a variety of modern-day diseases such as cancer, rheumatoid arthritis and diabetes. With many more candidate IgG's in the biopharmaceutical development pipeline the pressure to accurately understand and develop these candidates becomes more and more important.
