This study highlights the benefits of incorporating Recombinant Insulin in hiPSC culture to enhance the quality and productivity of these cells while maintaining their stability. The addition of Recombinant Insulin to the culture media resulted in improved cell morphology, proliferation, and attachment, indicating better cell culture performance. These improvements were observed even at a low insulin concentration (5 mg/L). Moreover, the pluripotency markers remained unaltered following the addition of Recombinant Insulin, indicating that it does not affect the stability of hiPSCs in culture. These findings suggest that using Recombinant Insulin as an ingredient in the formulation of cell culture media can improve the consistency and reliability of hiPSC production, ultimately enhancing the quality of regenerative medicine drug products.
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