5 Key Takeaways

• 1

  The INSTALL system utilizes circular single-stranded DNA (cssDNA) to reduce immune responses during genome editing.

• 2

  Traditional double-stranded DNA (dsDNA) templates activate immune sensors, limiting their use in genome editing.

• 3

  INSTALL achieves high-fidelity DNA integration with improved efficiency and tolerability in human cells and mice.

• 4

  Lipid nanoparticle delivery of cssDNA allows for systemic dosing and repeat administration without severe immune activation.

• 5

  INSTALL combines immune-evasive nucleic acids with recombinase precision, enhancing non-viral in vivo genome writing.