5 Key Takeaways

• 1

  Researchers at the University of Pennsylvania developed an optimized workflow for producing CAR T cells with large lentiviral transgenes.

• 2

  The new protocol improves transduction efficiency for constructs exceeding 10 kilobases, addressing a significant manufacturing challenge.

• 3

  Transduction efficiency increased by 10- to 12-fold for a 10.1 kb construct, with T-cell transduction rates rising up to 14.8-fold.

• 4

  The modified protocol supports robust T-cell expansion without toxicity and eliminates the need for cell sorting.

• 5

  This workflow could reduce manufacturing costs and be adapted for other immune cell types, expanding therapeutic applications.