Objective:

To review the application of Raman spectroscopy and its enhanced variants in detecting pandemic-prone viruses, emphasizing its significance in pandemic preparedness.

Key Findings:

• Raman-based methods can rapidly detect influenza and identify SARS-CoV-2 with high accuracy.
• Multiplexed detection of viruses in complex samples is achievable.
• Research focus is skewed towards accessible viruses, neglecting high-risk pathogens like Lassa and Marburg.

Interpretation:

While Raman spectroscopy shows promise in virology, significant technical challenges such as weak signal intensity and regulatory hurdles hinder its clinical application.

Limitations:

• Weak signal intensity and fluorescence interference limit detection capabilities.
• Lack of standardization affects reproducibility.
• Biosafety constraints restrict research on high-risk pathogens, impacting overall research focus.

Conclusion:

Raman spectroscopy has potential for pandemic preparedness but requires overcoming technical barriers and regulatory challenges to be effective in real-world diagnostics.