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Confident peptide mapping and disulfide bond analysis of an IgG2 monoclonal antibody

Authors:

Sara Carillo and Jonathan Bones
NIBRT – The National Institute for
Bioprocessing Research and Training,
Dublin, Ireland
[email protected] 
Tom Buchanan, Thermo Fisher Scientific

Key benefits

  • Complete sequence coverage peptide map of monoclonal antibody
  • Confident detection of inter- and intrachain disulfide bonds
  • Sensitive detection of low-level post-translational modifications (PTMs) with excellent mass accuracy

Goal

Demonstrate the utility and capability of the Thermo Scientific™ Orbitrap Exploris™ 240 mass spectrometer for the routine characterization of biotherapeutics through LC-MS peptide mapping. Provide the analyst with full sequence coverage, high confidence in low level PTM assignment through high-resolution accurate-mass (HRAM) MS/MS identification and overall ease in the interpretation of complex data.

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