Revolutionize Biosimilar Screening Processes with nanoDSF

Perform quick and meaningful screenings with minimal time and material requirements to select biosimilar candidates

Introduction

The development of biosimilars requires extensive physicochemical characterization of biosimilar candidate molecules which should match the quality profile of the reference molecule (originator).

Here we use nanoDSF (miniaturized differential scanning fluorimetry), a novel method of thermal unfolding profiling, to rapidly screen a variety of Fc-fusion protein biosimilar candidates. This study shows that this technology has the potential to revolutionize similarity screening processes in biosimilar profiling. The best-in-class precision, reproducibility and data quality of the NanoTemper Technologies Prometheus nanoDSF instruments delivers high-quality thermal unfolding profiles which show even smallest differences between biosimilar variants. Thus, nanoDSF allows for the ranking of biosimilar candidates according to the comparability of their unfolding profiles to the reference molecule.