Discovery & Development Drug Delivery, Analytical Science

Screening, Identifying, and Quantifying Potential Genotoxic Compounds with High Resolution LC/MS

Analysis of chlorhexidine drug substance using an Agilent 6545 Accurate Mass Q-TOF System and MassHunter Mass Profiler Software.

02/11/2016

This study demonstrates a routine screening of drugs to identify and quantify potential genotoxic compounds. In this Application Note, we used an Agilent 6545 Q-TOF LC/MS system to acquire accurate mass data of samples containing chlorhexidine as the drug substance. Agilent MassHunter Mass Profiler software was used to mine the data and compare different samples to generate a differential list of compounds. An accurate mass database search against the differential list identified 4-chloroaniline, a potential genotoxic compound. All Ions MS/MS acquisition mode was used to confirm 4-chloroaniline by MS/MS library matching, and quantify it using external standards. This workflow is suitable for batch-to-batch sample analysis for detecting and quantifying known potential genotoxic compounds.

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