Out with the Old, in with the New?
Not quite. Tried and tested analytical approaches like ELISA aren’t going away in the world of bioprocessing, but modern techniques allow for real-time analysis. Together, they present an orthogonal approach.
Alex Perieteanu |
There have been increasing calls from my company’s global clients for bio-layer interferometry (BLI) – a real-time analytical technique for studying biomolecular interactions – to be incorporated as a routine quality control test in bioprocessing. At the moment, BLI is most commonly used in the research phase for high-throughput target screening, but I believe it is more than applicable for quality control and to ensure that products are consistent and stable from lot to lot. BLI can measure interactions between many different types of molecules, whether a pair of proteins (or multiple proteins), a protein and a small molecule or peptide, or even two different fragments of DNA. Light of a particular wavelength is emitted through a fibre optic probe or biosensor. The probes have a unique chemistry, and multiple different chemistries are available commercially. When a molecule of interest binds to the coated probe there is a measurable change in the wavelength of the light, giving the ability to monitor on and off binding kinetics in real-time.
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