Subscribe to Newsletter

Characterization of Recombinant Protein Biotherapeutics by UHPLC-SEC-MALS

INTRODUCTION

Biotherapeutics are generally larger molecules such as peptides, proteins and monoclonal antibodies with monomer molecular weight ranging from 3,000-150,000 Da. The drug must remain free from impurities such as fragment, dimer and other higher order aggregates as they may cause severe immunogenic response. This becomes even more important if the biotherapeutic protein is thermally susceptible. Historically, size exclusion is the preferred mode of chromatography used for separation and characterization of such applications. Here we report the online detection of absolute molecular weight of two recombinant protein samples using a UHPLC size exclusion chromatography (SEC) column directly connected to the LenS3TM Multi-Angle Light Scattering (MALS) detector.

Read the full article now

Log in or register to read this article in full and gain access to The Medicine Maker’s entire content archive. It’s FREE!

Login
Receive content, products, events as well as relevant industry updates from The Medicine Maker and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Register to The Medicine Maker

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Medicine Maker magazine

Register