Subscribe to Newsletter

Thermal unfolding of antibodies

Comparison of nanoDSF and µDSC for thermal stability assessment during biopharmaceutical formulation development

Abstract

Assessing the thermal stability parameters of biologics is an integral part of formulation development in biopharmaceutical research. We compared two methods that detect thermal unfolding transition temperatures (Tm) of a therapeutic monoclonal antibody (mAb): nanoDSF, an advanced differential scanning fluorimetry method, which analyzes changes in the fluorescence emission properties of proteins, and differential scanning calorimetry (µDSC), which detects changes in the heat capacity of a protein solution upon unfolding. While both nanoDSF and µDSC provided precise and consistent data, the high-throughput and low sample consumption qualify nanoDSF as the ideal technology for thermal stability screening in biopharmaceutical development.

Read the full article now

Log in or register to read this article in full and gain access to The Medicine Maker’s entire content archive. It’s FREE!

Login
Receive content, products, events as well as relevant industry updates from The Medicine Maker and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Register to The Medicine Maker

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Medicine Maker magazine

Register