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Use of NanoSight Sample Assistant to increase throughput and reproducibility in the physical characterization of exosomes

Introduction

Nanosized plasma membrane vesicles secreted by cells into bodily fluids are known to have an important role in intracellular communication. These little messengers, called exosomes or extracellular vesicles (EVs), are undergoing intensive research to prove their potential uses within diagnostics and as therapeutics. This research often involves characterization of a large number of exosome samples. Reliable physical characterization throughout the exosomes development workflow is crucial to fully understanding their formation, pathways, stability and responses to changing conditions.

NanoSight Sample Assistant - Overview

Over the last few years, accompanying the exponential growth in exosomes research, NanoSight Nanoparticle Tracking Analysis (NTA) data has become an essential element of EV physical characterization. NTA-generated size and concentration data supports scientists through various processes in the exosomes characterization workflow, including purification, stability and storage studies. Promising discoveries, from early-stage fundamental research through to clinical trials, direct research towards understanding exosomes pathways. These studies are always accompanied by monitoring of the changes in the physical characteristics of samples. As growth in exosomes research accelerates, so the demand for quick, robust and accurate data continues to increase.

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