Subscribe to Newsletter
Manufacture Business Practice, Clinical Trials, Drug Delivery, Ingredients, Formulation, Quality & Compliance, Small Molecules, Trends & Forecasts

Utilization of kinITC in AFFINImeter

sponsored by Malvern Panalytical


It is commonly thought that ITC is a typical thermodynamic technique that is not really suited to extract kinetic information. It is thus commonplace to oppose ITC to SPR since the latter is a kinetic technique par excellence. However, this is at best an oversimplification since ITC is based upon kinetic measurements. Indeed, the raw signal measured in any ITC experiment is a heat power (in µJ s-1 or µcal s-1), that is essentially the rate of heat production, and not the heat itself evolved in a reaction. Obviously, this rate of heat production is directly related to the kinetics of the reaction taking place in the measurement cell, which is the reason why a microcalorimeter is potentially much more than merely a ‘heat-meter’. This is in line with the common observation that there are systems showing after each injection a quick return to baseline, that is a quick equilibration time, and others, on the contrary, showing slow, and even very slow, return to baseline. It should not come as a surprise, therefore, that ITC has already been used to derive kinetic information. Many readers, however, will probably be surprised to learn that the first “compensation-mode” calorimeter (the ancestor of the MicroCal VP-ITC and MicroCal ITC200 instruments) was devised in 1924 and used first to measure the heat power produced by flies [1, 2]. Therefore, the first “compensation-mode” calorimeter was used for what we now call a kinITC experiment.

Receive content, products, events as well as relevant industry updates from The Medicine Maker and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Register to The Medicine Maker

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Medicine Maker magazine