CAPTURING OF A scFv FROM E.COLI USING TOYOPEARL® AF-rPROTEIN L-650F
contributed by Tosoh |
An application example for the general method set-up and optimization
The optimization of the capture of single chain variable fragments on TOYOPEARL® Protein L resin using a robotic parallel chromatography system leads to significant purity improvement. More specifically, post-load washing with chaotropic agents can significantly reduce HCP content. It may further support on-column refolding of misfolded and aggregated product.
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