CAPTURING OF A scFv FROM E.COLI USING TOYOPEARL® AF-rPROTEIN L-650F

contributed by Tosoh | 10/03/2017

An application example for the general method set-up and optimization

Abstract

The optimization of the capture of single chain variable fragments on TOYOPEARL® Protein L resin using a robotic parallel chromatography system leads to significant purity improvement. More specifically, post-load washing with chaotropic agents can significantly reduce HCP content. It may further support on-column refolding of misfolded and aggregated product.

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