How to improve performance of the Protein A capturing step

contributed by Tosoh | 03/20/2017

Protein A has become a well-established platform for capturing of monoclonal antibodies and Fc-fusion proteins. Various Protein A resins have been commercialized, one with a higher dynamic binding capacity than the other. Especially the latest generation of Protein A resins was developed to bind large quantities of mAbs and Fc fusion proteins. One drawback of these resins is that host cell protein (HCP) clearance can be - depending on the feedstream - comparatively lower than for standard Protein A affinity resins. When aiming for a 2-step platform process, no compromises can be made with regards to HCP removal of the capturing step.

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