Cookies

Like most websites The Medicine Maker uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.

Measurements of protein electrophoretic mobility using DLS

Introduction

Zetasizer Nano is the market leader in dynamic and electrophoretic light scattering technology for measurements of hydrodynamic size and electrophoretic mobility. Dynamic Light Scattering (DLS) is a widely implemented technique for characterization of proteins and their formulations; allowing not only the determination of molecular size but also an assessment of stability. In recent years, the stability of proteins in solution has attracted significant scientific and commercial interest, due to the exponential growth in the use of proteins as biotherapeutic drugs. Since knowledge of formulation stability and behaviour are paramount for delivery of a safe and commercially viable product, the demand for tools that can characterize these formulation properties has increased. Protein mobility - measured using electrophoretic light scattering (ELS) - is one property that has been identified as a promising indicator of formulation stability, viscosity and behaviour [1].

Experimentally, protein mobility measurements present two practical challenges. (i) Working with protein solutions often means working with dilute concentrations. In DLS terms, this means working with a small amount of small-sized molecules that scatter proportionately low levels of light. Until now, most light scattering based instruments available on the market lack the sensitivity required for such measurements. (ii) Acquiring protein mobility measurements requires the application of an electric field to the sample - a physical process that itself can cause damage to the protein by stimulating aggregation [2]. Consequently, the resultant mobility measurements reflect that of the aggregate molecules rather than the protein of interest. Accurate and high quality measurements of protein mobility have three key requirements:

  1. An instrument with sufficient sensitivity to measure both the low count rates and low electrophoretic mobilities associated with dilute protein solutions.
  2. A measurement technique that reduces the risk of aggregate generation.
  3. A smart auto-measurement procedure that both recognizes and minimizes aggregate generation.
Figure 1: A. The Zetasizer Nano ZSP. B. Disposable folded capillary cells used for the diffusion barrier method.

Read the full article now

Log in or register to read this article in full and gain access to The Medicine Maker’s entire content archive. It’s FREE and always will be!

Login

Or register now - it’s free and always will be!

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Medicine Maker magazine
Register

Or Login via Social Media

By clicking on any of the above social media links, you are agreeing to our Privacy Notice.

Related White Papers

Performance validation of ScatterX78 against NIST reference materials

| Contributed by Malvern Panalytical

A Fast and High Precision Influenza Vaccine Potency Assay

| Contributed by ForteBio

MDRS used to confirm the pharmaceutical equivalence of Oral Solid Dose (OSD) products

| Contributed by Malvern Panalytical

Newsletter

Send me the latest from The Medicine Maker.

Sign up now

Register to The Medicine Maker

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Medicine Maker magazine

Register