Simple and Effective Method for Purification of DMT-ON Oligonucleotides using HIC Resins

contributed by Tosoh | 10/05/2020

INTRODUCTION

The use of synthetic oligonucleotide therapeutics continues to grow because of their effectiveness in the treatment of devastating diseases. Within the biopharmaceutical industry, oligonucleotide drug pipelines have increased significantly, along with the need for purification techniques of these highly valuable materials.

Dimethoxytrityl (DMT), a 5’ protecting group, is used in the synthesis of oligonucleotides to temporarily mask the characteristic chemistry of a 5’-hydroxy functional group. In many preparations of delicate oligonucleotides, DMT may be left on an oligonucleotide following synthesis to give stability to the molecule during subsequent processing.

Log in or register to read this article in full and gain access to The Medicine Maker’s entire content archive. It’s FREE!

Email*

Choose a password*

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

I want to stay up to date with the "Small Molecule" field I want to stay up to date with the Cell and Gene field I want to stay up to date with the Bioprocessing field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.