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Viruses and Viral Vaccines

Characterization by Nanoparticle Tracking Analysis

Abstract

As Nanoparticle Tracking Analysis (NTA) allows suspensions of nanoparticles to be visualized, sized and enumerated based on a particle-by-particle basis, its ability to determine the concentration and direct number frequency based particle size distribution profile means that virus preparations in particular can be studied in higher detail. This white paper discusses this application in more detail.

Nanoparticle Tracking Analysis (NTA) Overview

NTA utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension. A laser beam is passed through the sample chamber, and the particles in suspension in the path of this beam scatter light in such a manner that they can easily be visualized via a 20x magnification microscope onto which is mounted a camera. The camera, which operates at approximately 30 frames per second (fps), captures a video file of the particles moving under Brownian motion within the field of view of approximately 100 μm x 80 μm x 10 μm (Figure 1).

Figure 1: Schematic of the optical configuration used in NTA.

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