Tosoh

Modern chromatography resins were evaluated for the purification of a bispecific antibody.

The strong cation exchange resin TOYOPEARL Sulfate-650F offers high salt-tolerance, wide working pH range, and high dynamic binding capacity.

Antibody-drug conjugates (ADCs) are promising anti- cancer biopharmaceuticals with one of the highest annual growth rates. Four ADCs received market approval to this point.

TOYOPEARL NH2-750F is a salt-tolerant anion exchange resin well known for being capable of aggregate removal in both flow-through and bind / elute modes. Here, we demonstrate that the salt-tolerant TOYOPEARL NH2-750F is a very effective resin for the removal of endotoxin in a flow-through chromatography mode as well.

More potent formats of monoclonal antibodies (mAbs), such as bispecific antibodies (bsAbs), are on the rise in the area of biotherapeutics.

Purification schemes for monoclonal antibodies typically consist of three chromatographic steps accompanied by filtration steps.

An application example for the general method set-up and optimization

There is a growing interest in the development of continuous biomanufacturing systems to increase productivity and reduce costs.

TOYOPEARL Sulfate-650F cation exchange resin can be used for the removal of aggregates, host cell proteins and leached protein A in a single post Protein A mAb polishing step.

Protein A has become a well-established platform for capturing of monoclonal antibodies and Fc-fusion proteins.